Comparison of efficiency of various DNA extraction methods from cysts ofGiardia intestinalismeasured by PCR and TaqMan real time PCR
نویسندگان
چکیده
منابع مشابه
Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA.
AIM To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum s...
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Real-time PCR analysis of bacterial DNA isolated from faecal specimens has become increasingly used for the quantification of indigenous intestinal microbiota. The success of such analysis requires effective methods for the extraction of faecal bacterial DNA. Three extraction methods were assessed for their effectiveness in extracting Escherichia coli and Enterococcus spp. bacterial DNA from hu...
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Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
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The most important restriction for the detection in water samples is the low concentration of Giardia intestinalis cysts, additional difficulty is the presence of PCR inhibitors. We have carried out trials in order to assess the sensitivity of semi-nested PCR and TaqMan real time PCR on the basis of DNA extracted from G. intestinalis cysts coming from spiked environmental and distilled water sa...
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Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
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ژورنال
عنوان ژورنال: Parasite
سال: 2010
ISSN: 1252-607X,1776-1042
DOI: 10.1051/parasite/2010174299